Device

Part:BBa_K1491035:Design

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-26)


Constitutive ER Intein T7 RNAP with YFPH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2950
    Illegal AgeI site found at 2914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2425


Design Notes

Constitutive ER Intein T7 RNAP with YFP for quantification of T7 RNAP


Source

The promoter is BBa_J23115, the RBS is BBa_B0034, the T7 RNA polymerase is BBa_K145001, the ER Intein is BBa_K1491024, the YFP is BBa_K864100

References